Analytical Chemistry recently published “Aqueous Two-Phase System Rehydration of Antibody-Polymer Microarrays Enables Convenient Comparmentalized Multiplex Immunoassays.”
This article, authored by Lisa Eiden, Cameron Yamanishi, Shuichi Takayama, and John F. Dishinger, details improvements the PHASIQ team made to the technology. This streamlined protocol reduces steps in the assay preparation.
From the abstract:
Aqueous two-phase systems (ATPS) exploiting the phase-separating polymers poly(ethylene) glycol (PEG) and dextran (DEX) have been used to compartmentalize antibodies and prevent cross-talk in multliplex, plate-based ELISA. However, the resulting protocol is tedious and lengthy, and requires too many user steps to be practical for widespread use. Here, we report an improved, user-friendly, cross-talk-free multiplex ELISA method in which dehydrated arrays of colocalized capture and detection antibodies in DEX are prepared on multiwell plates.
Eiden, L.; Yamanishi, C.; Takayama, S.; Dishinger, JF. “Aqueous two-phase system rehydration of antibody-polymer microarrays enables convenient compartmentalized multiplex immunoassays” Anal Chem 2016 DOI: 10.1021/acs.analchem.6b02960.