PHASIQ’s unique solution for cross-talk-free multiplex ELISA has been recognized by awarded patents. Our experimental protocol was first published in Frampton et al. (2014). Since that time, the PHASIQ team has streamlined the published protocol for ease of use in Eiden et al. (2016).
PHASIQ technology in Scientific Publications
Frampton et al. (2014) describe how aqueous two-phase systems (ATPS) prevent antibody-antigen cross-reactions. Persistent dextran microdroplets confine capture and detection antibodies together. These microdroplets separate antibodies that might cross-react, preventing them entirely. In bath- or cocktail-format ELISA, antibody-antigen cross reactions can and do occur, resulting in false-positives and false-negatives.
Just Add Sample, Wash, Read
The Frampton paper describes the original lab protocol. After licensing the technology from the University of Michigan, PHASIQ has worked to streamline, simplify, and commercialize the invention. PHASIQ’s assay kit comes as a pre-made 96-well custom plate. It includes capture and detection antibodies in dehydrated spots. The procedure is easy and fast, requiring only the application of protein standards and samples prepared in our diluent buffer, followed by a wash protocol, streptavidin-HRP and substrate addition. Eiden et al. (2016) describes the streamlined protocol.
PHASIQ has exclusive worldwide licenses to US and PCT patents and pending patents for our core technology from the University of Michigan.
- Solution Microarrays and Uses Thereof US 8,969,256 B2 and Multiphase Microarrays and Uses Thereof WO 2011/116256 A2.
- Dehydrated Aqueous Polymer Solutions PCT/US2011/028941.
 J. P. Frampton, J. B. White, A. B. Simon, M. Tsuei, S. Paczesny, and S. Takayama, “Aqueous two-phase system patterning of detection antibody solutions for cross-reaction-free multiplex ELISA.,” Scientific reports, vol. 4, 2014.
 Eiden, L.; Yamanishi, C.; Takayama, S.; Dishinger, JF. “Aqueous two-phase system rehydration of antibody-polymer microarrays enables convenient compartmentalized multiplex immunoassays” Anal Chem 2016 DOI: 10.1021/acs.analchem.6b02960.